منابع مشابه
Environmentally Sensitive Protein Reagents*
The use of protein reagents which exhibit sensitivity to changes in molecular environment has provided a means of examining certain properties of enzymes during interaction with their substrates (1). The recently reported 2-hydroxy-5nitrobenzyl bromide provided one such reagent, the absorption spectrum of which showed sensitivity to environmental changes by virtue of the p-nitrophenolic groupin...
متن کاملStructure-based engineering of environmentally sensitive fluorophores for monitoring protein-protein interactions.
Single, extrinsic, environmentally sensitive fluorophores can be used to quantitate formation of protein-protein complexes. These can be prepared semi-synthetically by covalent coupling to single cysteine mutations introduced at positions where the fluorophore is predicted to respond to formation of the complex without adversely affecting the interaction. The three-dimensional structure of a pr...
متن کاملEnvironmentally-sensitive design: Leonardo WAS right!
The design of environmentally-sensitive products and manufacturing facilities is considered by some members of the industrial community to be one of the current frontiers of engineering practice, while others consider it to be another fad. Here today but gone tomorrow. This paper examines this topic from a biological stance by asserting that humankind’s activities should emulate nature’s flora ...
متن کاملL1 Elements as a Source of Environmentally Sensitive Genetic Instability
LINE-1 is the only active autonomous non-LTR human retroelement. The expression and activity of these elements contribute to human genomic instability. LINE-1 elements comprise 17% of the human genome, which translates into about 5x10 L1 copies, the majority of which are defective due to truncations at their 5’ end. We have analyzed the expression profiles of endogenous L1 elements from differe...
متن کاملAmino(oligo)thiophene-based environmentally sensitive biomembrane chromophores.
There is a growing need for cellular imaging with fluorescent probes that emit at longer wavelengths to minimize the effects of absorption, autofluorescence, and scattering from biological tissue. In this paper a series of new environmentally sensitive hemicyanine dyes featuring amino(oligo)thiophene donors have been synthesized via aldol condensation between a 4-methylpyridinium salt and vario...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1965
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)45235-5